FLOW CYTOMETRY FUNCTIONAL ASSAYS


Flow cytometry functional assay showing tumor model 1 out of 6 treated in vitro with therapeutic agents (T1,T2,T3,T4). The outcome of the functional assay study predicted tumor model 1 treated with combination T1+T2+T4 in vitro (top) would be efficacious in vivo (bottom).


Flow cytometry functional assay showing tumor model 2 out of 6 treated in vitro with therapeutic agents (T1,T2,T3,T4). The outcome of the functional assay study predicted tumor model 2 treated with combination T1+T2 in vitro (top) would be non-efficacious in vivo (bottom).

FLOW CYTOMETRY FUNCTIONAL ASSAYS

Immune and tumor cell proliferation assays

Cytokine and other intracellular protein analysis

Specificity assays for bi/tri-specific antibodies. ADCs or antibody dependent cell killing analysis

Receptor based signaling or intracellular phosphorylation and downstream signaling events

CAR T cell and gene therapy efficiency assays. CAR T cell proliferation, activation, exhaustion, and specificity tests.

Vaccine priming assays and immune reactions

Apoptosis, cellular death, and other methods specific for your drug MOA

Immune or tumor cell viability and function under specific metabolic conditions

BRDU, EDU, and others

Plug and play kits from various vendors

Screen for caspases using various kits

We have the ability to adapt to many assays depending on your specific needs


Flow cytometry in vitro functional assay showing the impact of amino acids or sugars on t cell proliferation. The percentage of amino acids or sugars was varied and compared to 100% and 0% concentrations in complete growth medium. Proliferation (x-axis) is shown as the number of cells (y-axis) in each division peak as the curve progresses from right to left along the x-axis.