FLOW CYTOMETRY PANEL DESIGN

Validation of flow cytometry multicolor panels are critical for accurate assessment of treatment impact on cell subsets of the blood, tissue, or tumor microenvironment. Factors such as sample collection and preparation, fixation and permeabilization reagents, and staining technique can influence the accuracy and precision of study data. As a result, even off the shelf flow cytometry panels from reputable vendors should be validated for use in your specific study protocol. Once the multicolor panel design is completed, data can be represented and visualized in t-SNE plots to view how specific cell subset organize.

Flow cytometry data analysis services


Distribution showing higher B cell frequencies compared to other healthy donors


Distribution showing wide separation between T cells compared to other healthy donors


Distribution showing lower CD8 T cell frequencies compared to other healthy donors


Distribution showing lower T cell frequencies compared to healthy donors

target antigen evaluation by flow cytometry


Immune cell subsets: CD4 T cells (red), CD8 T cells (green), B cells (purple), classical monocytes (olive green), non-classical monocytes (light green), intermediate monocytes (yellow green), and CD33+ non-monocytes (sky blue)


(red = highest expression tier, yellow = 2nd highest expression tier, light blue = 3rd highest expression tier, blue = 4th highest expression tier, and black = baseline or <)